abridged_biotech_sequence_amgen_1.pdf | |
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Purpose- make RFP(red fluorescent protein) from jelly fish in bacteria and learn about steps for genetic engineering
Materials and Procedure
2a: materials and procedure can be found in Amgen lab manual part 2A
4a: materials and procedure can be found in Amgen lab manual part 4a
5a: materials and procedure can be found in Amgen lab manual part 5a
6: materials and procedure can be found in Amgen lab manual part 6a
Experimental Overview
Part 2a: verification of plasmid by restriction digest
-cut plasmid with BamHI + HindIII to cutout RFP-ara from bacterial plasmid
Part 4a: verification of plasmid digest by electrophoresis
Part 5a: transformation of bacteria with recombinant plasmid
Part 6: purification of RFP using chromatography
Results
2a: able to tell all the components were there
4a: see picture
5a: for our purposes all the proteins reproduced
6: at the end we got our RFP to show
Analysis and Conclusion
The gene we got did not fully work properly, so the Red Flourescent Protein was not visible in the end.
Reflection
My group worked pretty well together. We each had our separate tasks and duties to get the whole lab done. The lab was a little bit confusing because we had some big gaps in between each part, which confused me quite a bit. This was pretty interesting because we used a lot of different tools and instruments. I thought it was neat to see the different techniques in using them. I could have worked a little better on being organized with my results and data.
Purpose- make RFP(red fluorescent protein) from jelly fish in bacteria and learn about steps for genetic engineering
Materials and Procedure
2a: materials and procedure can be found in Amgen lab manual part 2A
4a: materials and procedure can be found in Amgen lab manual part 4a
5a: materials and procedure can be found in Amgen lab manual part 5a
6: materials and procedure can be found in Amgen lab manual part 6a
Experimental Overview
Part 2a: verification of plasmid by restriction digest
-cut plasmid with BamHI + HindIII to cutout RFP-ara from bacterial plasmid
Part 4a: verification of plasmid digest by electrophoresis
Part 5a: transformation of bacteria with recombinant plasmid
Part 6: purification of RFP using chromatography
Results
2a: able to tell all the components were there
4a: see picture
5a: for our purposes all the proteins reproduced
6: at the end we got our RFP to show
Analysis and Conclusion
The gene we got did not fully work properly, so the Red Flourescent Protein was not visible in the end.
Reflection
My group worked pretty well together. We each had our separate tasks and duties to get the whole lab done. The lab was a little bit confusing because we had some big gaps in between each part, which confused me quite a bit. This was pretty interesting because we used a lot of different tools and instruments. I thought it was neat to see the different techniques in using them. I could have worked a little better on being organized with my results and data.